10 20 30 40 50 MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT 60 70 80 90 100 DEAAFQKLMS NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGFPDKQPRK K
Purity > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as single band (monomer) migrating at a molecular weight below 14.4 kDa.
Supplied as 1 mg/mL solution in Tris 20 mM, pH 7.5, NaCl 150 mM, CaCl2 5 mM, DTT 2 mM. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 2980 M-1cm-1 calculated).
Storage -20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles.