Description
Description
MW = 17.6 kDa calculated. Matrix Metalloproteinase-19 isoform 1 RASI-1 (MMP-19, Matrix Metalloproteinase RASI, MMP-18) catalytic domain cloned from human cDNA, expressed in E. coli. The enzyme consists of the catalytic domain of human MMP-19, (residues 101-256, UniProtKB accession Q99542).
Sequence
110 120 130 140
M- LGRWRKKHLT FRILNLPSTL PPHTARAALR QAFQDWSNVA
150 160 170 180 190
PLTFQEVQAG AADIRLSFHG RQSSYCSNTF DGPGRVLAHA DIPELGSVHF
200 210 220 230 240
DEDEFWTEGT YRGVNLRIIA AHEVGHALGL GHSRYSQALM APVYEGYRPH
250
FKLHPDDVAG IQALYG
Purity
> 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at a molecular weight between 14.4 and 18.4 kDa.
Supplied as
0.2 mg/mL solution in Tris 20 mM pH 7.2, CaCl
2 10 mM, ZnCl
2 0.1 mM, NaCl 0.3 M, acetohydroxamic acid (AHA) 0.2 M. The concentration is calculated by the analysis of the absorbance at 280 nm (ε
280 = 25440 M
-1cm
-1 calculated).
Specific activity
> 1.5 U/μg. Activity described as U=100 pmol/min at 25°C using a colorimetric ***** with thiopeptide Ac-Pro-Leu-Gly-[2- mercapto-4-methyl-pentanoyl]-Leu-Gly-OC2H5 (Biomol) as substrate.
Storage
-80°C. After initial defrost, aliquot the product into individual tubes and refreeze at -80°C.
Avoid repeated freeze/thaw cycles.