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Description MW = 8.5 kDa calculated. Calbindin D9K (Protein S100-G, CABP, Vitamin D-dependent calcium-binding protein, Intestinal) cloned from bovine cDNA, expressed in E. coli. The protein consists of Calbindin D9K, mutant P47M(residues 5-79, UniProtKB accession P02633) with 2 Ca2+ ions bound.
10 20 30 40 50 M-KSPEEL KGIFEKYAAK EGDPNQLSKE ELKLLLQTEF PSLLKGPSTL 60 70 DELFEELDKN GDGEVSFEEF QVLVKKISQ
Purity > 95% by SDS-PAGE. The protein is observed, in denaturing conditions, as a single band migrating at molecular weight below 14.4 kDa.
Supplied as 1.0 mg/mL solution in ammonium acetate 50 mM pH 6.0. The concentration is calculated by the analysis of the absorbance at 280 nm (ε280 = 1280 M-1cm-1 calculated). (unless otherwise specified, NMR tubes will be sold at a concentration of 1mM)
Characteristics Under the above described conditions, to avoid precipitation or protein dimerization, the product can be concentrated up to 2 mM.
Storage -20°C. The protein is stable at 4°C for at least 2 weeks and at 25°C for several hours. After initial defrost, aliquot the product into individual tubes and refreeze at -20°C. Avoid repeated freeze/thaw cycles. NMR USE: after the experiments return the sample at 4°C.